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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
19/12/2011 |
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Data da última atualização: |
23/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
WOHLRES-VIANA, S.; BOITE, M. C.; VIANA, J. H. M.; MACHADO, M. A.; CAMARGO, L. S. de A. |
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Afiliação: |
SABINE WOHLRES-VIANA, UFJF; Mariana Cortes Boite, UFF; JOAO HENRIQUE MOREIRA VIANA, CNPGL; MARCO ANTONIO MACHADO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Relative expression of mRNAs related to cavitation process in bovine embryos produced in vivo and in vitro. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Revista Brasileira de Zootecnia, v. 40, n. 1, p. 124-128, 2011. |
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DOI: |
https://doi.org/10.1590/S1516-35982011000100017 |
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Idioma: |
Inglês |
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Conteúdo: |
The objectives of this work were to identify and to evaluate possible differences on gene expression of aquaporins and Na/K-ATPases transcripts between embryos in vivo and in vitro produced. For each group, 15 blastocysts distributed in three pools were used for RNA extraction followed by amplification and reverse transcription. The resulting cDNAs were submitted to Real-Time PCR, using the GAPDH gene as endogenous control. It was not possible to identify AQP1 transcripts. Relative expression of AQP3 (1.33 ± 0.78) and AQP11 (2.00 ± 1.42) were not different in blastocysts in vitro and in vivo produced. Na/K-ATPase ?1 gene (2.25 ± 1.07) was overregulated whereas Na/K-ATPase ?2 transcripts 0.40 ± 0.30) did not differ among blastocysts produced in vitro from those produced in vivo. Transcripts for gene AQP1 are not present in bovine blastocysts. In vitro culture system does not alter expression of genes AQP3, AQP11 and Na/K-ATPase ?2 genes, however, it affects expression of Na/K-ATPase ?1. |
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Palavras-Chave: |
Blastocoel; Osmotic gradient; Water channel. |
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Thesaurus Nal: |
gene expression. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/50836/1/Artigo-meta-2011-RBZ-v40n1a17-JHenrique.pdf
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Marc: |
LEADER 01745naa a2200229 a 4500
001 1910325
005 2023-01-23
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/S1516-35982011000100017$2DOI
100 1 $aWOHLRES-VIANA, S.
245 $aRelative expression of mRNAs related to cavitation process in bovine embryos produced in vivo and in vitro.$h[electronic resource]
260 $c2011
520 $aThe objectives of this work were to identify and to evaluate possible differences on gene expression of aquaporins and Na/K-ATPases transcripts between embryos in vivo and in vitro produced. For each group, 15 blastocysts distributed in three pools were used for RNA extraction followed by amplification and reverse transcription. The resulting cDNAs were submitted to Real-Time PCR, using the GAPDH gene as endogenous control. It was not possible to identify AQP1 transcripts. Relative expression of AQP3 (1.33 ± 0.78) and AQP11 (2.00 ± 1.42) were not different in blastocysts in vitro and in vivo produced. Na/K-ATPase ?1 gene (2.25 ± 1.07) was overregulated whereas Na/K-ATPase ?2 transcripts 0.40 ± 0.30) did not differ among blastocysts produced in vitro from those produced in vivo. Transcripts for gene AQP1 are not present in bovine blastocysts. In vitro culture system does not alter expression of genes AQP3, AQP11 and Na/K-ATPase ?2 genes, however, it affects expression of Na/K-ATPase ?1.
650 $agene expression
653 $aBlastocoel
653 $aOsmotic gradient
653 $aWater channel
700 1 $aBOITE, M. C.
700 1 $aVIANA, J. H. M.
700 1 $aMACHADO, M. A.
700 1 $aCAMARGO, L. S. de A.
773 $tRevista Brasileira de Zootecnia$gv. 40, n. 1, p. 124-128, 2011.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| 2. |  | FERREIRA, G. B.; SANTOS, F. C. dos; ALBUQUERQUE FILHO, M. R. de; BARBOSA, C. A.; BIANCINI, L.; BRUGNERA, P.; BREDA, C. E.; REIS, K. A.; FUMAGALLI, F. P.; S. FILHO, B. O.; SILVA, R. A.; N. JÚNIOR, D. R.; PEDROSA, M. B.; SANTOS, J. B. dos. Determinação dos efeitos de dose, fontes de gesso (mineral e fosfogesso) e frequência de aplicação sobre a produtividade de culturas em sucessão no cerrado da Bahia. In: ENCONTRO TÉCNICO CÍRCULO VERDE, 4., 2009, Luís Eduardo Magalhães. Resultados de pesquisa da Círculo Verde no Oeste da Bahia - safra 2008/2009: anais. Luís Eduardo Magalhães, BA: Círculo Verde Assessoria Agronômica & Pesquisa, 2009. p. 63-89. (Círculo Verde. Comunicado Técnico, 3).| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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